Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton.
Identifieur interne : 000F98 ( Main/Exploration ); précédent : 000F97; suivant : 000F99Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton.
Auteurs : Yu Zhong [Japon] ; Yoshiyuki Yoshinaka ; Tadahiro Takeda ; Noriko Shimizu ; Sayaka Yoshizaki ; Yoshio Inagaki ; Shinobu Matsuda ; Gisho Honda ; Nobutaka Fujii ; Naoki YamamotoSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Agents antiVIH.
- métabolisme : Polygonum.
- pharmacologie : Agents antiVIH, Médicaments issus de plantes chinoises.
- Fermentation, Lignée cellulaire, Polygonum, Réplication virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Anti-HIV Agents.
- chemical , pharmacology : Anti-HIV Agents, Drugs, Chinese Herbal.
- chemistry : Polygonum.
- drug effects : HIV-1, Virus Replication.
- metabolism : Polygonum.
- Cell Line, Fermentation.
Abstract
A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (IIIB) infection in MT-4 cells with EC50 values of 0.5 microg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 microg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC 50 of 11.56 microg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 microg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 degrees C in 1N HCl or 1N NaOH, and after treatment with 100 mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.
DOI: 10.1016/j.antiviral.2005.02.003
PubMed: 15911029
Affiliations:
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Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name></author><author><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name></author><author><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name></author><author><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name></author><author><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name></author><author><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name></author><author><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name></author><author><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></author></analytic><series><title level="j">Antiviral research</title><idno type="ISSN">0166-3542</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-HIV Agents (isolation & purification)</term><term>Anti-HIV Agents (pharmacology)</term><term>Cell Line</term><term>Drugs, Chinese Herbal (pharmacology)</term><term>Fermentation</term><term>HIV-1 (drug effects)</term><term>Polygonum (chemistry)</term><term>Polygonum (metabolism)</term><term>Virus Replication (drug effects)</term></keywords><keywords 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xml:lang="fr"><term>Agents antiVIH</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Polygonum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Polygonum</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Agents antiVIH</term><term>Médicaments issus de plantes chinoises</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Fermentation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fermentation</term><term>Lignée cellulaire</term><term>Polygonum</term><term>Réplication virale</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (IIIB) infection in MT-4 cells with EC50 values of 0.5 microg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 microg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC 50 of 11.56 microg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 microg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 degrees C in 1N HCl or 1N NaOH, and after treatment with 100 mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Zhong, Yu" sort="Zhong, Yu" uniqKey="Zhong Y" first="Yu" last="Zhong">Yu Zhong</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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